Phenotyping - Blood - Lymphocyte Subsets
REQUESTING AND COLLECTING

Ordering:
When required urgently for ATG treatment - indicate ATG on request

form. Same day service available.

Collection:
1 full tube each of Acid-Citrate-Dextrose (yellow top) and EDTA (purple

top). EDTA can be shared with haematology if FBC requested.
Container(s):
Acid-Citrate-Dextrose (6 mL yellow-top) - Required

EDTA (5 mL purple-top) - Required

More than one tube type required

GENERAL INFORMATION

Test use:
For use in investigating and monitoring immunodeficiencies. NOT for

investigation of leukaemias and lymphomas.

Availability:
Test performed office hours (Mon-Sat). Results available 2 days after

sample arrival in laboratory.

Reference Intervals:
CD2 73 - 94 %

E-rosette+ 1310 - 2080 x 10^6/L

CD3 59 - 83 %

Total T-cells 780 - 2600 x 10^6/L

CD3+CD4+ 30 - 57 %

Helper T-cells 500 - 1650 x10^6/L

CD3+CD8+ 14 - 35 %

Cytotox T cells 280 - 600 x10^6/L

CD3+HLADR+ 2 - 13%

Active T cells not established

CD19 5 - 20 %

Total B cells 80 - 300 x10^6/L

CD3-CD16/56+ 2 - 29 %

NK cells 70 - 280 x10^6/L

Laboratory:
Flow Lab (8382-9176)

Patient Results:
(02) 8382-9100
Synonyms:
CD2 / CD3 for ATG

CD2 lymphycytes

CD3 lymphocytes

Lymphocyte phenotyping / subsets

Natural Killer cell Count

T and B cells

T cell phenotyping
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INSTRUCTIONS FOR REFERRING LABORATORIES
Handling & Transport:
Store and transport whole blood at room temperature
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INSTRUCTIONS FOR HANDLING AT SYDPATH
SydPath Handling:
SPRO: Processing by laboratory only. Leave at room temperature.

Send EDTA to Haematology for FBC. If not requested add FBC.

Data Entry Code:
SUB (plus C23 if ATG, CD2 or CD3 indicated)

SydPath Division:
Immunopathology

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Date/Time published: 30/03/2020 17:35:12
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