Department of Anatomical Pathology

Fluorescence In Situ Hybridisation (FISH)
on Paraffin Sections

Important Notes:

• Paraffin FISH analysis is not funded by Medicare and costs $325 per test. The referring laboratory will be billed unless other arrangements have been made. We will not directly bill patients unless we have their signed agreement to pay (download Patient Payment Agreement form). Enquiries about this, or other aspects of the test should be directed to Dr Adrienne Morey on (02) 8382 9218

• A specific request form is required. This can be downloaded in pdf format here FISH request form.

• HER-2 FISH in patients with metastatic breast cancer and equivocal HER-2 immunohistochemistry is still funded by Roche. In future other breast cancers will be triaged by HER-2 CISH.

FISH probes currently held in SydPath:

Breast Cancer Probes

HER-2 / cep17 dual colour probe (HER-2 CISH probe)

Neuro-oncology Probes

1p / 1q, 19q / 19p dual colour probe sets

EGFR / cep7 dual colour probe

Soft tissue Probes

FKHR (13q14) dual colour break apart probe

SYT (18q11.2) dual colour break apart probe

EWSR1 (22q12) dual colour break apart probe

NMYC (2p24.1) locus specific probe

CHOP (12q13) dual colour break apart probe

Lymphoma Probes

ALK (2p23) dual colour break apart probe

BCL6 (3q27) dual colour break apart probe

MYC (8q24) dual colour break apart probe

IGH/MYC,CEP8 t(8;14)(q24;q32) dual colour dual fusion probe

IGH (14q32) dual colour break apart probe

MALT (18q21) dual colour break apart probe

IGH/BCL2 t(14;18)(q32;q21) dual colour dual fusion probe

IGH/CCND1 t(11;14)(q13;q32) dual colour dual fusion probe

Other Haematology Probes
(please contact the Cytogenetics Department regarding applications for these probes)

EGR1/D5S23,D5S721 (5q31/5p15.2) dual colour probe mix

IGH/FGFR3 t(4;14)(p16.3;q32) dual colour dual fusion probe

CSF1R/ D5S23,D5S721 (5q33-34/5p15.2) dual colour probe mix

CEP 8 (8p11.1-q11.1) centromeric enumeration probe

AML1/ETO t(8;21)(q22;q22) dual colour dual fusion probe

BCR/ABL t(9;22)(q34;q11.2) dual colour dual fusion probe

LSI p53/LSI ATM (17p13.1/11q22.3) and LSI 13q34/CEP 12 multi-colour probe sets

MLL (11q23) dual colour break apart probe

TEL/AML1 t(12;21)(p13;q22) dual colour dual fusion probe

RB1 (13q14) locus specific probe

D13S319 (13q14.3) locus specific probe

IGH/MAF t(14;16)(q32;q23) dual colour dual fusion probe

p53(17p13.1) locus specific probe

RARA (17q21.1) dual colour break apart probe

PML/RARA t(15;17)(q22;q21.1) dual colour dual fusion

CBFB (16q22) dual colour break apart probe

CEPX/Y (Xp11.2-q11.2,Yq12) centromeric enumeration probe mix

Other Probes

Centromeric enumeration probes

CEP 3

CEP 7

CEP 8

CEP 12

Locus specific probes

LSI 21 (21q22.13-q22.2)

Whole chromosome paint probes

WCP 2

WCP 8

WCP 9

WCP 11

WCP 17

WCP 20

WCP 21